Impact of Virgin Coconut Oil and Carvedilol on Neurobehaviour, Apoptotic and Inflammatory Brain Markers in Doxorubicin Treated Mice

Abstract

One of the significant side effects of doxorubicin treatment for cancer patients is cognitive impairment, and the mechanisms underlying this impairment must be investigated to treat or prevent it. The current study investigates the impact of virgin coconut oil and carvedilol administration on neurobehaviour brain apoptotic and inflammatory markers in doxorubicin-treated mice. 32 male and 32 female mice were randomly assigned to four groups of 8 animals each, and the treatment lasted for twenty-eight days. Group 1 animals in both the male and female groups were the controls. In comparison, the Group 2 animals in both groups received doxorubicin dosage (3.75mg/kg body weight) intraperitoneally weekly as a single dose on days 5, 12, 19 and 26 to make up for the desired amount (15 mg/kg body weight). Group 3 animals received doxorubicin and were orally treated with virgin coconut oil (5ml/kg body weight) for 28 days. Group 4 animals received doxorubicin and were treated with carvedilol (5 mg/kg body weight) weekly for three days (days 5-7 for four weeks). The histological analysis of the brain tissue was done by staining the tissues with haematoxylin and eosin. The data was analyzed using GraphPad Prism 9.0. Analysis of Variance (ANOVA) was used to compare between groups. All results were presented as mean±SEM. Both virgin coconut oil and carvedilol demonstrated ameliorative effects on neurobehaviour and apoptotic and inflammatory brain markers.